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Ein neuartiger Assay zur Messung chemotaktischer Migration in Echtzeit

A novel assay for the measurement of chemotactic migration in real time
 
: O’Clair, L.; Ellinger, B.; Kalmer, M.; Szybut, C.; Borsetti, Y.

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Biospektrum 23 (2017), Nr.2, S.171-173
ISSN: 0947-0867
ISSN: 1868-6249
Deutsch, Englisch
Zeitschriftenaufsatz
Fraunhofer IME ()

Abstract
Traditional techniques for studying chemotaxis, such as Boyden chamber assays, are limited due to their endpoint nature and inability to visualize cells. A novel technique is presented that generates kinetic, automated, image-based measurements and movies of chemotaxis in a reproducible 96-well format. This technique offers the advantages of visual confirmation of migration and morphology, reduced cell requirement and the use of biologically relevant surface substrates, with or without fluorescent labels.

: http://publica.fraunhofer.de/dokumente/N-503196.html