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2017
Journal Article
Titel
Ein neuartiger Assay zur Messung chemotaktischer Migration in Echtzeit
Alternative
A novel assay for the measurement of chemotactic migration in real time
Abstract
Traditional techniques for studying chemotaxis, such as Boyden chamber assays, are limited due to their endpoint nature and inability to visualize cells. A novel technique is presented that generates kinetic, automated, image-based measurements and movies of chemotaxis in a reproducible 96-well format. This technique offers the advantages of visual confirmation of migration and morphology, reduced cell requirement and the use of biologically relevant surface substrates, with or without fluorescent labels.