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Low dose carbon black nanoparticle exposure does not aggravate allergic airway inflammation in mice irrespective of the presence of surface polycyclic aromatic hydrocarbons

 
: Lindner, Karina; Webering, Sina; Ströbele, Michael; Bockhorn, Henning; Hansen, Tanja; König, Peter; Fehrenbach, Heinz

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Volltext urn:nbn:de:0011-n-4976209 (1.7 MByte PDF)
MD5 Fingerprint: 373c0bea951f616d7666489adce1feb0
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Erstellt am: 4.7.2018


Nanomaterials 8 (2018), Nr.4, Art. 213, 18 S.
ISSN: 2079-4991
Englisch
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer ITEM ()
Asthma; allergic airway inflammation; Mucociliary clearance; carbon black nanoparticle; Polycyclic aromatic hydrocarbons; exacerbation

Abstract
Exposure to exogenous noxae, such as particulate matter, can trigger acute aggravations of allergic asthma-a chronic inflammatory airway disease. We tested whether Carbon Black nanoparticles (CBNP) with or without surface polycyclic aromatic hydrocarbons (PAH) aggravate an established allergic airway inflammation in mice. In an ovalbumin mouse model, Printex((R))90 (P90), P90 coated with benzo[a]pyrene (P90-BaP) or 9-nitroanthracene (P90-9NA), or acetylene soot exhibiting a mixture of surface PAH (AS-PAH) was administered twice (70 microL, 100 microg/mL) during an established allergic airway inflammation. We analyzed the immune cell numbers and chemokine/cytokine profiles in bronchoalveolar lavages, the mRNA expressions of markers for PAH metabolism (Cyp1a1, 1b1), oxidative stress (HO-1, Gr, Gpx-3), inflammation (KC, Mcp-1, IL-6, IL-13, IL-17a), mucin synthesis (Muc5ac, Muc5b), the histology of mucus-producing goblet cells, ciliary beat frequency (CBF), and the particle transport speed. CBNP had a comparable primary particle size, hydrodynamic diameter, and zeta-potential, but differed in the specific surface area (P90 > P90-BaP = P90-9NA = AS-PAH) and surface chemistry. None of the CBNP tested increased any parameter related to inflammation. The unmodified P90, however, decreased the tracheal CBF, decreased the Muc5b in intrapulmonary airways, but increased the tracheal Muc5ac. Our results demonstrated that irrespective of the surface PAH, a low dose of CBNP does not acutely aggravate an established allergic airway inflammation in mice.

: http://publica.fraunhofer.de/dokumente/N-497620.html