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Sortase-mediated ligation of purely artificial building blocks

: Dai, Xiaolin; Mate, Diana M.; Glebe, Ulrich; Garakani, Tayebeh Mirzaei; Körner, Andrea; Schwaneberg, Ulrich; Böker, Alexander

Volltext urn:nbn:de:0011-n-4941228 (2.6 MByte PDF)
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Erstellt am: 17.5.2018

Polymers. Online resource 10 (2018), Nr.2, Art. 151, 13 S.
ISSN: 2073-4360
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer IAP ()

Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP–polymer, NP–NP, and polymer–polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction—the conserved peptide LPETG—and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.