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Recombinant collagen I peptide microcarriers for cell expansion and their potential use as cell delivery system in a bioreactor model

: Suarez Muñoz, Melva; Confalonieri, Davide; Walles, Heike; Dongen, Elisabeth M.W.M. van; Dandekar, Gudrun


Journal of visualized experiments : JoVE. Online resource 132 (2018), Art. e57363
ISSN: 1940-087X
Fraunhofer ISC ()
tissue engineering; microcarrier; Zelle

We propose a cell expansion protocol on macroporous microcarriers and their use as delivery system in a perfusion bioreactor to seed a decellularized tissue matrix. We also include different techniques to determine cell proliferation and viability of cells cultured on microcarriers. Furthermore, we demonstrate functionality of cells after bioreactor cultures.