Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Confining Brownian motion of single nanoparticles in an ABELtrap

: Dienerowitz, M.; Heitkamp, T.; Gottschall, T.; Limpert, J.; Börsch, M.


Andrews, D.L. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Complex Light and Optical Forces XI : 31 January-2 February 2017, San Francisco, California, United States
Bellingham, WA: SPIE, 2017 (Proceedings of SPIE 10120)
ISBN: 978-1-5106-0681-4
ISBN: 978-1-5106-0682-1
Paper 1012017
Conference "Complex Light and Optical Forces" <11, 2017, San Francisco/Calif.>
Fraunhofer IOF ()

Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap1-7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object's Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.