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Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

: Rizzo, Riccardo; Alvaro, Maria; Danz, Norbert; Napione, Lucia; Descrovi, Emiliano; Schmieder, Stefan; Sinibaldi, Alberto; Chandrawati, Rona; Rana, Subinoy; Munzert, Peter; Schubert, Thomas; Maillart, Emmanuel; Anopchenko, Aleksei; Rivolo, Paola; Mascioletti, Alessandro; Sonntag, Frank; Stevens, Molly M.; Bussolino, Federico; Michelotti, Francesco


Sensors and Actuators. B 255 (2018), Pt.2, S.2143-2150
ISSN: 0925-4005
European Commission EC
FP7-ICT; 318035; BILOBA
Bloch electromagnetic surface wave Bio-sensors for early cancer diagnosis
Fraunhofer IWS ()
Fraunhofer IOF ()
Biosensor platform; Bloch surface wave; Label-free optical sensor; Enhanced fluorescence; Tumor biomarker; Complex matrice

We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.