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Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC)

: Sellrie, F.; Lenz, C.; Andersson, A.; Wilhelmsson, L.M.; Schenk, J.A.


Talanta. The international journal of pure and applied analytical chemistry 124 (2014), S.67-70
ISSN: 0039-9140
Fraunhofer IBMT ()

We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA.