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Microfluidic system for in-vitro hypoxia assays

 
: Busek, Mathias; Grünzner, Stefan; Steege, Tobias; Steinfelder, C.; Schmieder, Florian; Klotzbach, Udo; Sonntag, Frank

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Volltext urn:nbn:de:0011-n-4359136 (1.3 MByte PDF)
MD5 Fingerprint: 8040178711b0e047019aad17844b6056
Copyright Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Erstellt am: 30.5.2017


Gray, B.L. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Microfluidics, BioMEMS, and Medical Microsystems XV : San Francisco, California, United States, 28 - 30 January 2017
Bellingham, WA: SPIE, 2017 (Proceedings of SPIE 10061)
Paper 1006110, 10 S.
Conference "Microfluidics, BioMEMS, and Medical Microsystems" <15, 2017, San Francisco/Calif.>
Bundesministerium für Wirtschaft und Technologie BMWi
Plasmafügen
Englisch
Konferenzbeitrag, Elektronische Publikation
Fraunhofer IWS ()
hypoxia; cell culture; Lab-on-a-Chip; Perfusion; μPIV; Microfluidics

Abstract
Hereby presented is a microfluidic system, including a micro pump, an oxygenator and a cell culture chamber for perfusion controlled hypoxia assays. It consists of laser-structured polycarbonate (PC) foils and an elastomeric membrane which were joined together using thermal diffusion bonding. The elastomer forms an oxygenator element. The microfluidic system is characterized using non-invasive flow measurement based on micro-Particle-Image- Velocimetry (μPIV) and optical oxygen measurement utilizing the oxygen dependent fluorescence decay. Based on those experimental results and mathematical considerations, the oxygenator and mass transport phenomena within the microfluidic system can be described. This oxygen sensor, the micro pump, a controlling device and the gas mixture at the oxygenator forms a regulatory circuit to adjust the oxygen content in the cell culture chamber and helps to produce well-defined hypoxic conditions for the cells.

: http://publica.fraunhofer.de/dokumente/N-435913.html