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Candida guilliermondii as a potential biocatalyst for the production of long-chain α,ω-dicarboxylic acids

: Werner, Nicole; Dreyer, Miriam; Wagner, Wenke; Papon, Nicolas; Rupp, Steffen; Zibek, Susanne


Biotechnology letters 39 (2017), Nr.3, S.429-438
ISSN: 0141-5492 (Print)
ISSN: 1573-6776 (Online)
Fraunhofer IGB ()
Acyl-CoA oxidase; cytochrome P450 hydroxylase; genetic engineering; long chain; dicarboxylic acid; oleic acid; β-Oxidation; ω-Oxidation

Objectives: To explore Candida guilliermondii for the production of long-chain dicarboxylic acids (DCA), we performed metabolic pathway engineering aiming to prevent DCA consumption during β-oxidation, but also to increase its production via the ω-oxidation pathway.
Results: We identified the major β- and ω-oxidation pathway genes in C. guilliermondii and performed first steps in the strain improvement. A double pox disruption mutant was created that slowed growth with oleic acid but showed accelerated DCA degradation. Increase in DCA production was achieved by homologous overexpression of a plasmid borne cytochrome P450 monooxygenase gene.
Conclusion: C. guilliermondii is a promising biocatalyst for DCA production but further insight into its fatty acid metabolism is necessary.