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The biochemical origins of the surface-enhanced Raman spectra of bacteria: a metabolomics profiling by SERS

 
: Premasiri, W.R.; Lee, J.C.; Sauer-Budge, A.; Théberge, R.; Costello, C.E.; Ziegler, L.D.

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Analytical and bioanalytical chemistry 408 (2016), Nr.17, S.4631-4647
ISSN: 1618-2642 (Print)
ISSN: 1618-2650 (Online)
Englisch
Zeitschriftenaufsatz
Fraunhofer CMI ()

Abstract
The dominant molecular species contributing to the surface-enhanced Raman spectroscopy (SERS) spectra of bacteria excited at 785 nm are the metabolites of purine degradation: adenine, hypoxanthine, xanthine, guanine, uric acid, and adenosine monophosphate. These molecules result from the starvation response of the bacterial cells in pure water washes following enrichment from nutrient-rich environments. Vibrational shifts due to isotopic labeling, bacterial SERS spectral fitting, SERS and mass spectrometry analysis of bacterial supernatant, SERS spectra of defined bacterial mutants, and the enzymatic substrate dependence of SERS spectra are used to identify these molecular components. The absence or presence of different degradation/salvage enzymes in the known purine metabolism pathways of these organisms plays a central role in determining the bacterial specificity of these purine-base SERS signatures.

: http://publica.fraunhofer.de/dokumente/N-422943.html