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SPARC (secreted protein acidic and rich in cysteine) of the intestinal nematode Strongyloides ratti is involved in mucosa-associated parasite-host interaction

 
: Anandarajah, Emmanuela M.; Ditgen, Dana; Hansmann, Jan; Erttmann, Klaus D.; Liebau, Eva; Brattig, Norbert W.

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Molecular and Biochemical Parasitology 207 (2016), Nr.2, S.75-83
ISSN: 0166-6851
Englisch
Zeitschriftenaufsatz
Fraunhofer IGB ()
sparc; excretory/secretory products; strongyloides; immune response; 3d-cell culture; caenorhabditis-elegans; helminth infection; endothelial-cells; epithelial-cells; glycoprotein; Matrix; responses; immunity; model; osteonectin

Abstract
The secreted protein acidic and rich in cysteine (SPARC), found in the excretory/secretory products of Strongyloides ratti, is most strongly expressed in parasitic females. Since SPARC proteins are involved in the modulation of cell-matrix interactions, a role of the secreted S. ratti SPARC (Sr-SPARC) in the manifestation of the parasite in the host’s intestine is postulated. The full-length cDNA of Sr-SPARC was identified and the protein was recombinantly expressed. The purified protein was biologically active, able to bind calcium, and to attach to mucosa-associated human cells. Addition of Sr-SPARC to an in vitro mucosal three-dimensional-cell culture model led to a time-dependent release of the cytokines TNF-α, IL-22, IL-10 and TSLP. Of importance, exposure with Sr-SPARC fostered wound closure in an intestinal epithelial cell model. Here, we demonstrate for the first time that SPARC released from the nematode is a multifunctional protein affecting the mucosal immune system.

: http://publica.fraunhofer.de/dokumente/N-421936.html