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Rapid detection of bacteria from blood with surface-enhanced Raman spectroscopy

: Boardman, A.K.; Wong, W.S.; Premasiri, W.R.; Ziegler, L.D.; Lee, J.C.; Miljkovic, M.; Klapperich, C.M.; Sharon, A.; Sauer-Budge, A.F.


Analytical chemistry 88 (2016), Nr.16, S.8026-8035
ISSN: 0003-2700
ISSN: 1520-6882
Fraunhofer CMI ()

Traditional methods for identifying pathogens in bacteremic patients are slow (24-48+ h). This can lead to physicians making treatment decisions based on an incomplete diagnosis and potentially increasing the patient's mortality risk. To decrease time to diagnosis, we have developed a novel technology that can recover viable bacteria directly from whole blood and identify them in less than 7 h. Our technology combines a sample preparation process with surface-enhanced Raman spectroscopy (SERS). The sample preparation process enriches viable microorganisms from 10 mL of whole blood into a 200 μL aliquot. After a short incubation period, SERS is used to identify the microorganisms. We further demonstrated that SERS can be used as a broad detection method, as it identified a model set of 17 clinical blood culture isolates and microbial reference strains with 100% identification agreement.