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Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis

 
: Righetti, Francesco; Nuss, Aaron M.; Twittenhoff, Christian; Beele, Sascha; Urban, Kristina; Will, Sebastian; Bernhart, Stephan H.; Stadler, Peter F.; Dersch, Petra; Narberhaus, Franz

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Proceedings of the National Academy of Sciences of the United States of America : PNAS 113 (2016), Nr.26, S.7237-7242
ISSN: 0027-8424
ISSN: 1091-6490
Englisch
Zeitschriftenaufsatz
Fraunhofer IZI ()
RNA structure; RNA thermometer; temperature dependence; translation control; virulence

Abstract
RNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 ° C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 ° C, 37 ° C, and 42 ° C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5?-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAswindow into the immense complexity of human brain function.

: http://publica.fraunhofer.de/dokumente/N-404935.html