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Steroid treatment reduces allergic airway inflammation and does not alter the increased numbers of dendritic cells and calcitonin gene-related peptide-expressing neurons in airway sensory ganglia

: Le, Duc Dung; Funck, Ulrike; Wronski, Sabine; Heck, Sebastian; Tschernig, Thomas; Bischoff, Markus; Sester, Martina; Herr, Christian; Bals, Robert; Welte, Tobias; Braun, Armin; Dinh, Quoc Thai


Neuroimmunomodulation 23 (2016), Nr.1, S.18-26
ISSN: 1021-7401
ISSN: 1423-0216
Deutsche Forschungsgemeinschaft DFG
DI 1429/2-1
Fraunhofer ITEM ()
Dendritic cells; calcitonin gene-related peptide; jugular-nodose ganglion complex; neuroimmune interaction; steroid treatment

Objectives: Our previous data demonstrated that allergic airway inflammation induces migration of dendritic cells (DC) into airway sensory jugular and nodose ganglia (jugular-nodose ganglion complex; JNC). Here we investigated the effects of steroid treatment regarding the expression and migration of DC and calcitonin gene-related peptide (CGRP)-immunoreactive neurons of vagal sensory ganglia during allergic airway inflammation.
Methods: A house dust mite (HDM) model for allergic airway inflammation was used. The mice received 0.3 mg fluticasone propionate per kilogram of body weight in the last 9 days. JNC slices were analyzed on MHC II, the neuronal marker PGP9.5, and the neuropeptide CGRP.
Results: Allergic airway inflammation increased the numbers of DC and CGRP-expressing neurons in the JNC significantly in comparison to the controls (DC/neurons: HDM 44.58 +/- 1.6% vs. saline 33.29 +/- 1.6%, p < 0.05; CGRP-positive neurons/total neurons: HDM 30.65 +/- 1.9% vs. saline 19.49 +/- 2.3%, p < 0.05). Steroid treatment did not have any effect on the numbers of DC and CGRP-expressing neurons in the JNC compared to HDM-treated mice.
Conclusions: The present findings indicate an important role of DC and CGRP-containing neurons in the pathogenesis of allergic airway inflammation. However, steroid treatment did not have an effect on the population of DC and neurons displaying CGRP in the JNC, whereas steroid treatment was found to suppress allergic airway inflammation.