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Universal lab-on-a-chip platform for complex, perfused 3D cell cultures

 
: Sonntag, Frank; Schmieder, Florian; Ströbel, Joachim; Grünzner, Stefan; Busek, Mathias; Steege, Tobias; Polk, Christoph; Klotzbach, Udo; Günther, K.

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Volltext urn:nbn:de:0011-n-3829048 (11 MByte PDF)
MD5 Fingerprint: e5e384706885d5784fe3012e2161c692
Copyright Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Erstellt am: 16.4.2016


Gray, B.L. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Microfluidics, BioMEMS, and Medical Microsystems XIV : 13 - 15 February 2016, San Francisco, California
Bellingham, WA: SPIE, 2016 (Proceedings of SPIE 9705)
Paper 970516
Conference "Microfluidics, BioMEMS, and Medical Microsystems" <14, 2016, San Francisco/Calif.>
Englisch
Konferenzbeitrag, Elektronische Publikation
Fraunhofer IWS ()
Lab-on-a-Chip; lasers; liver; medical diagnostics; multilayers; oxygen; particles; printing; rapid manufacturing; skin equivalent

Abstract
The miniaturization, rapid prototyping and automation of lab-on-a-chip technology play nowadays a very important role. Lab-on-a-chip technology is successfully implemented not only for environmental analysis and medical diagnostics, but also as replacement of animals used for the testing of substances in the pharmaceutical and cosmetics industries. For that purpose the Fraunhofer IWS and partners developed a lab-on-a-chip platform for perfused cell-based assays in the last years, which includes different micropumps, valves, channels, reservoirs and customized cell culture modules. This technology is already implemented for the characterization of different human cell cultures and organoids, like skin, liver, endothelium, hair follicle and nephron. The advanced universal lab-on-a-chip platform for complex, perfused 3D cell cultures is divided into a multilayer basic chip with integrated micropump and application-specific 3D printed cell culture modules. Moreover a technology for surface modification of the printed cell culture modules by laser micro structuring and a complex and flexibly programmable controlling device based on an embedded Linux system was developed. A universal lab-on-a-chip platform with an optional oxygenator and a cell culture module for cubic scaffolds as well as first cell culture experiments within the cell culture device will be presented. The module is designed for direct interaction with robotic dispenser systems. This offers the opportunity to combine direct organ printing of cells and scaffolds with the microfluidic cell culture module. The characterization of the developed system was done by means of Micro-Particle Image Velocimetry (μPIV) and an optical oxygen measuring system.

: http://publica.fraunhofer.de/dokumente/N-382904.html