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Bio-orthogonal immobilization of fibroblast growth factor 2 for spatial controlled cell proliferation

: Lühmann, Tessa; Jones, Gabriel; Gutmann, Marcus; Rybak, Jens-Christoph; Nickel, Joachim; Rubini, Marina; Meinel, Lorenz


ACS biomaterials science & engineering 1 (2015), Nr.9, S.740-746
ISSN: 2373-9878
Fraunhofer IGB ()
fibroblast growth factor 2 (FGF-2); bio-orthogonal immobilization; genetic codon expansion; decoration; proliferation

Presentation of therapeutic proteins on material surfaces is challenged by random immobilization chemistries through lysine or cysteine residues, typically leading to heterogeneous product outcome. Pharmaceutical quality standards warrant a controlled process ideally through site specific conjugation. Therefore, we deployed genetic codon expansion to engineer a propargyl-l-lysine (Plk)-modified FGF-2 analogue, enabling site-specific copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Site-specific decoration of Plk-FGF-2 to particles sparked cell proliferation of human osteosarcoma cells in a spatially controlled manner around the decorated carrier, rendering this approach instrumental for the future design of quality-improved bioinstructive scaffold outcome.