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Biovasc-term (R): A platform technology to engineer of vascularised human tissues

: Walles, H.; Steinke, M.; Ruecker, C.; Hansmann, J.; Groeber, F.; Blunk, T.; Metzger, M.; Dandekar, G.; Pullig, O.

Tissue Engineering. Part A 21 (2015), Supplement 1, S.247
ISSN: 1937-3341
ISSN: 1937-335X
Tissue Engineering International & Regenerative Medicine Society (TERMIS World Congress) <4, 2015, Boston/Mass.>
Konferenzbeitrag, Zeitschriftenaufsatz
Fraunhofer IGB ()

Vascularization is a major challenge in creating tissues ex vivo. Complex tissue engineered constructs exceeding a thickness of 100–200 mikrometer need a vascular system in order to supply the cells with oxygen and remove waste products. This restricts generation of tissues with an appropriate size for clinical application. We developed 3D vascularized tissues based on decellularized porcine small bowl segments and preserved tubular structures of the capillary network within the collagen matrix functional associated with one small vein and artery (biological vascularized scaffold - BioVaScR). This vascularized matrix enables the generation of a functional artificial vascular network and vascularized tissues as trachea1, bone2, skin3, fatty tissue4, intestine5, and tumour6. Vascularized tissues are applied as tissue models in preliminary stage to animal experiments for investigations of functional parameters like penetration, distribution and metabolization of substances in different tissue layers. With the help of suitable markers, issues concerning proliferation, differentiation, cell death, but also, the initiation and graduation of tumors of the applied cell types can be examined. Furthermore, first clinical application will be shown during the presentation. Non-invasive monitoring of such tissues during their in vitro maturation or post implantation is relevant for graft evaluation. However, traditional methods to analyses cell and matrix components in tissue engineered constructs such as histology, immunohistochemistry or biochemistry requires invasive tissue processing, resulting in scarification of these constructs. At the end of the presentation an overview of non-destructive methods as impedance7 and Raman8 spectroscopy to characterize the complex implants will be given.