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2014
Conference Paper
Titel
Microfluidic flow reactors with integrated micro-heaters and fluorescent temperature sensors for reaction monitoring
Abstract
We present the combined integration of fluorescent temperature sensor layers and resistive micro-heaters in microfluidic reactor chips and their application for temperature control and monitoring during an on-chip enzymatic conversion. The production process of the chips consists of wet chemical etching, photolithography, blade coating and soft lithography. These multifunctional chips allow both temperature control and microscopic imaging of the local temperature in microfluidic flow reactors. They are thus able to determine the temperature dependency of chemical and biological reaction on the microscale. This is demonstrated by the tryptic cleavage of modified peptides yielding a fluorescent product at different temperatures.