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2014
Conference Paper
Titel
Integration of fluorescent pH sensors in microfluidic free-flow isoelectric focusing platforms using automated inkjet printing
Abstract
In this work, we present the integration of fluorescent pH sensor arrays, into microfluidic free-flow electrophoresis (FFE) chips. The sensor cocktail consisted a fluorescein derivate, which was linked to a hydrophilic acrylate based polymer. Optical pH sensor arrays were generated by automated inkjet printing and integrated into FFE platforms using a fabrication cycle based on multiple photopolymerisation. The functionality of microfluidic free-flow isoelectric focusing ((FFIEF) platforms was demonstrated in separations of three P503 labeled proteins via monitoring of sample and sensor fluorescence in different spectral channels. These multifunctional microanalytical devices allow nondestructive online monitoring of isoelectric points (pl).