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Development of hematological and immunological characteristics in neonatal rats

: Sewald, Katherina; Müller, Meike; Buschmann, Jochen; Hansen, Tanja; Lewin, Geertje


Reproductive toxicology 56 (2015), S.109-117
ISSN: 0890-6238
ISSN: 1873-1708
European Teratology Society (ETS Annual Conference) <43, 2015, Amsterdam>
Zeitschriftenaufsatz, Konferenzbeitrag
Fraunhofer ITEM ()
juvenile animal study; development; blood; hematopoietic system; immune system

As major immunological and hematological parameters evolve during the early period of life, laboratory data must be interpreted in relation to developmental changes. Wistar (WU) rats were sacrificed on PND2, 4, 7, 10, 14, 17 and 21. Peripheral blood, bone marrow, thymus samples and spleen cells were collected and a broncheoalveolar lavage (BAL) performed. Parameters of blood counts changed considerably between time points. IgM and IgG levels steadily increased. Spontaneous spleen cell proliferation was low before PND21, although mitogens had stimulatory effects above baseline. In the spleen, T-lymphocyte counts tripled by PND17 (mainly attributed to CD8+ cytotoxic T-cells and CD4+ T-helper cells). In peripheral blood an increase in B-lymphocytes to about 60% of the cell number was observed. In BAL fluid, macrophages represented 95-98% of the cells. In thymus architecture, lymphoblast migration was seen and epithelial structures appeared. The data presented will help to distinguish between maturational changes and treatment-related effects.