Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Nanoprobing the acidification process during intracellular uptake and trafficking

 
: Lerch, Simone; Ritz, Sandra; Bley, Karina; Messerschmidt, Claudia; Weiss, Clemens K.; Musyanovych, Anna; Landfester, Katharina; Mailänder, Volker

:

Nanomedicine. Nanotechnology, biology and medicine 11 (2015), Nr.6, S.1585-1596
ISSN: 1549-9642
ISSN: 1549-9634
Englisch
Zeitschriftenaufsatz
Fraunhofer ICT-IMM ()
nanoparticle; intracellular trafficking; acidification; pH responsiveness; Rab-family

Abstract
Many nanoparticular drug delivery approaches rely on a detailed knowledge of the acidification process during intracellular trafficking of endocytosed nanoparticles (NPs). Therefore we produced a nanoparticular pH sensor composed of the fluorescent pH-sensitive dual wavelength dye carboxy seminaphthorhodafluor-1 (carboxy SNARF-1) coupled to the surface of amino-functionalized polystyrene NPs (SNARF-1-NP). By applying a calibration fit function to confocal laser scanning microscopy (CLSM) images, local pH values were determined. The acidification and ripening process of endo/lysosomal compartments containing nanoparticles was followed over time and was found to progress up to 6 h to reach an equilibrium pH distribution (maximum pH 5.2 [± 0.2]). The SNARF-1-NP localization in endo/lysosomal compartments was confirmed by transmission electron microscopy (TEM) and quantitative co-localization analysis with fluorescent endolysosomal marker Rab-proteins by confocal laser scanning microscopy (CLSM). The herein described nanoparticular pH-sensor is a versatile tool to monitor dynamic pH processes inside the endolysosomal compartments.

: http://publica.fraunhofer.de/dokumente/N-345532.html