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Method and kit for determining the genome integrity and/or the quality of a library of DNA-sequences otained by deterministic restriction site whole genome amplification

 
: Klein, Christoph Andreas; Polzer, Bernhard Michael; Manaresi, Nicolo

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Frontpage ()

EP 20130195770 : 20131204
Englisch
Patent, Elektronische Publikation
Fraunhofer ITEM ()

Abstract
The present invention relates to a method for determining the integrity of the genome of a sample and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification (DRS-WGA) of the genome of the sample comprising the steps of: (a) providing the library of DNA sequences; (b) amplifying the library of DNA sequences by PCR using at least one first primer pair which hybridises to a DNA sequence of the library having a length from 1000 bp to 5000 bp and corresponding to a sequence of the genome located on a first chromosome arm, the step of amplifying giving rise to a first PCR product from 50 to 1000 bp; (c) detecting the first PCR product; (d) correlating the presence of the first PCR product with the integrity of the genome and/or the quality of the library of DNA sequences. The present invention further relates to a related kit and uses thereof.

: http://publica.fraunhofer.de/dokumente/N-345480.html