Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Untersuchung des Abbaus von Bisphenol A und Epoxidharzen durch Enzyme des Mikroorganismus Trametes versicolor

 
: Zell, Sarah

Biberach, 2014, 59 S.
Biberach, Hochschule, Bachelor Thesis, 2014
Deutsch
Bachelor Thesis
Fraunhofer IWU ()
Bisphenol A; laccase; trametes versicolor; Epoxidharz

Abstract
This thesis deals with the biodegradability of epoxy resins and bisphenol A. Biodegradability depends on the selected microorganism and its enzymes. The experiments were carried out with the fungi Trametes versicolor.
The background of this study is the biodegradability of carbon fiber reinforced plastics. Carbon fiber reinforced plastics consist mostly of epoxy resins and carbon fibers. The most used reactant of epoxy resins is bisphenol A.
Epoxy resins draw an analogy to the biopolymer lignin. For that reason the ligninolytic enzymes laccase and manganese peroxidase were used in this study. These enzymes can be produced by the microorganism Trametes versicolor.
The degradations of bisphenol A and the epoxy resins were analyzed. Araldite and Poly(bisphenol A- co-epichlorohydrin) served as epoxy resins. The paper answers the question if the selected ligninolytic enzymes are able to degrade epoxy resins and bisphenol A. It also deals with the question if Trametes versicolor is able to degrade bisphenol A.
To proof the degradation two analytical methods have been selected. The utilized methods for detection are high performance liquid chromatography and thin-layer chromatography.
The analyses show, that bisphenol A, Araldite and Poly(bisphenol A- co-epichlorohydrin) can be broken by the enzyme laccase. There is no biodegradation by the enzyme manganese peroxidase.
The fungi Trametes versicolor is able to produce the enzyme laccase in the presence of bisphenol A. The production of laccase was proofed by a sodium dodecyl sulfate polyacrylamide gel electrophoresis. There was also a decrease in the concentration of bisphenol A.

: http://publica.fraunhofer.de/dokumente/N-322156.html