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Lung tissue culture model for assessment of cytotoxicity and inflammation induced by inhalable carbon black nanoparticles

: Braun, Armin

The Toxicologist 53 (2014), Nr.1, S.514-515, PS 1953
ISSN: 0731-9193
Society of Toxicology (Annual Meeting) <53, 2014, Phoenix/Ariz.>
Fraunhofer ITEM ()

Carbon black nanoparticles (CBNP) are produced in several megatons per year and are used in the industry mainly in tires, inks and for stabilization of elastomers. CBNP have a large surface area to volume ratio and can be covered with polycyclic aromatic hydrocarbon (PAH). CBNP were evaluated as possible cancerogenetic. However, it is remain unclear whether the effects are caused by PAH known to be cancerogenic or by CBNP itself. This study focused on evaluation of one CBNP without PAH (Printex® 90), two CBNP with only one PAH (Printex® 90 Benzo[a]pyrene and Printex® 90 9-Nitroanthracene) as well as one covered with different PAH (acetylene soot). Precision cut lung slices (PCLS) were used as acute exposure model.
PCLS (murine, rat and human) were exposed to different concentrations of Printex® 90, acetylene soot, Printex® 90 Benzo[a]pyrene and Printex® 90 Nitroanthracene for 24 h. The size distribution of stable particle suspension in DMEM medium was determined by dynamic light scattering. Viability of PCLS was assessed by LIVE/DEAD® staining and determination of metabolic activity using the WST-1 assay. Pro-inflammatory immune responses were assessed in tissue lysate and quantified using ELISA (e.g. IL-1).