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Freeze-drying of HI-6-loaded recombinant human serum albumin nanoparticles for improved storage stability

 
: Dadparvar, M.; Wagner, S.; Wien, S.; Worek, F.; Briesen, H. von; Kreuter, J.

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European journal of pharmaceutics and biopharmaceutics 88 (2014), Nr.2, S.510-517
ISSN: 0939-6411
ISSN: 1873-3441
Englisch
Zeitschriftenaufsatz
Fraunhofer IBMT ()

Abstract
Severe intoxications with organophosphates require the immediate administration of atropine in combination with acetyl cholinesterase (AChE) reactivators such as HI-6. Although this therapy regimen enables the treatment of peripheral symptoms, the blood-brain barrier (BBB) restricts the access of the hydrophilic antidotes to the central nervous system which could lead to a fatal respiratory arrest. Therefore, HI-6-loaded albumin nanoparticles were previously developed to enhance the transport across this barrier and were able to reactivate organophosphate-(OP)-inhibited AChE in an in vitro BBB model. Since HI-6 is known to be moisture-sensitive, the feasibility of freeze-drying of the HI-6-loaded nanoparticles was investigated in the present study using different cryo- and lyoprotectants at different concentrations. Trehalose and sucrose (3%, w/v)-containing formulations were superior to mannitol concerning the physicochemical parameters of the nanoparticles whereas trehalose-containing samples were subject of a prolonged storage stability study at temperatures between -20. °C and +40. °C for predetermined time intervals. Shelf-life computations of the freeze-dried HI-6 nanoparticle formulations revealed a shelf-life time of 18. months when stored at -20. °C. The formulations' efficacy was proven in vitro by reactivation of OP-inhibited AChE after transport over a porcine brain capillary endothelial cell layer model.

: http://publica.fraunhofer.de/dokumente/N-301885.html