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Warm or cold ischemia in animal models of lung ischemia-reperfusion injury

Is there a difference?
: Warnecke, G.; Sommer, S.P.; Gohrbandt, B.; Fischer, S.; Hohlfeld, J.M.; Niedermeyer, J.; Haverich, A.; Struber, M.


The Thoracic and cardiovascular surgeon 52 (2004), Nr.3, S.174-179
ISSN: 0171-6425
Fraunhofer ITEM ()
ischemia-reperfusion injury; animal model; LPD solution; Ischemia; Transplantation

Objective: Experiments were designed to compare large animal models for lung ischemia-reperfusion injury using either 90 minutes' warm or 24 hours' cold ischemia.
Methods: In 6 pigs, the left lung was perfused in situ with cold LPD solution. Reperfusion was started after 90 min of warm ischemia. The left lung of 6 donor pigs was perfused with LPD solution and the lung transplanted after 24 hours of cold storage into 6 recipient pigs. In both groups the right pulmonary artery and main bronchus were clamped after reperfusion of the left lung. Bronchoalveolar lavage (BAL) was obtained before ischemia and after 2 h of reperfusion. Hemodynamic and respiratory parameters were monitored. Surfactant activity was determined from the BAL in a pulsating bubble surfactometer.
Results: Moderate lung injury was evident after reperfusion, but without any significant differences between groups. Surfactant composition and function were mildly impaired after reperfusion in both groups.
Conclusions: Our results suggest that short periods of warm ischemia might be accepted as an accelerated model for ischemia-reperfusion injury of the lung after cold storage.