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Advances in the quantification of protein microarrays

: Pratsch, K.; Wellhausen, R.; Seitz, H.


Current opinion in chemical biology 18 (2014), S.16-20
ISSN: 1367-5931
ISSN: 1879-0402
Fraunhofer IBMT ()

The determination of the protein composition is a challenging task, yet a valuable endeavor. Information that lies within the composition can help predicting pathogenic mechanisms and diseases. Therefore, a mandatory requirement is the accurate quantification. Protein microarrays are a promising alternative to complement mass spectrometry (MS) techniques. Nevertheless, effects in microarray production and assay development may lead to altered signal intensities. Techniques like Norma Curve provide a more robust quantification by taking the immobilized protein content into account. Printing dilution series can be used to construct a non-linear standard curve to estimate the amount of bound IgG, which helps to overrule the detection range of one order of magnitude. Standard protocols based on projects like the 'minimum information about a proteomics experiment' will help to improve quality and overall comparability.