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CD26 (dipeptidyl-peptidase IV)-dependent recruitment of T cells in a rat asthma model

 
: Kruschinski, C.; Skripuletz, T.; Bedoui, S.; Tschernig, T.; Pabst, R.; Nassenstein, C.; Hörsten, S. von

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Clinical and experimental immunology 139 (2005), Nr.1, S.17-24
ISSN: 0009-9104
ISSN: 0964-2536
Englisch
Zeitschriftenaufsatz
Fraunhofer ITEM ()
Brown Norway rat; Fischer F344 rat; Lewis rat; CD4+ T lymphocyte; Eosinophils; T-Lymphocytes; Rats

Abstract
CD26 truncates several chemokines as well as neuropeptides and influences immune responses via modulation of cell adhesion and T cell activation, suggesting an involvement of CD26 in asthmatic and airway inflammation. Therefore, Fischer 344 (F344), Brown Norway (BN) and Lewis (LEW) rat strains, which differ in their CD26-like enzymatic activity, were compared using an asthma model. Additionally, two CD26-deficient mutant F344 rat substrains were included and compared to the wild-type F344 substrain. Immunization was performed twice with ovalbumin (OVA), and 2 weeks later the rats were challenged with OVA intratracheally Flow cytometry (FACS) analysis of different leucocyte subsets as well as enzyme-linked immunosorbent assay (ELISA) for IgE levels in the blood and bronchoalveolar lavage (BAL) were performed 24 h after challenge. LEW rats with the lowest CD26 activity among the rat strains investigated here displayed significantly reduced CD4+ T cell numbers in the BAL compared to wild-type F344 and BN rats. Moreover, in asthma, the ratio of CD26+ to CD26- T cell receptor (TCR)-positive cells increased significantly in F344 and LEW but not BN rats. Most intriguingly, in both CD26-deficient F344 rat substrains the number of CD4+ T lymphocytes was markedly reduced compared to wild-type F344. The decrease in T cell recruitment observed in the CD26-deficient rats was associated with significantly reduced OVA-specific IgE-titres. This is the first report to show a remarkably reduced T cell recruitment in rat strains that either lack or exhibit reduced CD26-like enzymatic activity, suggesting a role for CD26 in the pathogenesis of asthma via T cell-dependent processes such as antibody production.

: http://publica.fraunhofer.de/dokumente/N-27416.html