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Nanoporous aluminum oxide as a novel support material for enzyme biosensors

: Heilmann, A.; Teuscher, N.; Kiesow, A.; Janasek, D.; Spohn, U.


Journal of nanoscience and nanotechnology 3 (2003), Nr.5, S.375-379
ISSN: 1533-4880
Fraunhofer IWM ()
anodic oxidation; nanoporous alumina; enzyme sensor; amperometric detection

To construct novel amperometric sensors for the detection of hydrogen peroxide and pyruvate, peroxidase and pyruvate oxidase were immobilized in self-supporting nanoporous alumina membranes those made by anodic oxidation. Pyruvate oxidase and other enzymes were enclosed in poly(carbamoylsulfonate) hydrogel and sucked into the nanoporous alumina structure before polymerization. The alumina membranes were investigated by scanning electron microscopy before and after the enzyme immobilization. In an amperometric flow detector cell, pyruvate and hydrogen peroxide were detected under flow injection analysis conditions in concentration ranges from 1 µM to 100 µM and 5 µM to 500 µM, respectively. The achieved operational stability showed that alumina membranes can be used to construct enzyme-modified electrodes.