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Targeted delivery of dendritic polyglycerol-doxorubicin conjugates by scFv-SNAP fusion protein suppresses EGFR(+) cancer cell growth

: Hussain, A.F.; Kruger, H.R.; Kampmeier, F.; Weissbach, T.; Licha, K.; Kratz, F.; Haag, R.; Calderon, M.; Barth, S.


Biomacromolecules 14 (2013), Nr.8, S.2510-2520
ISSN: 1525-7797
ISSN: 1526-4602
Fraunhofer IME ()

Development of effective polymer-based nanocarriers for the successful application in cancer therapy still remains a great challenge in current research. In the present study we present a dendritic polyglycerol-based multifunctional drug immunoconjugate that specifically targets and kills cancer cell lines expressing epidermal growth factor receptor (EGFR). The nanocarrier was provided with a dendritic core as a multifunctional anchoring point, doxorubicin (Doxo) coupled through a pH-sensitive linker, a fluorescence marker, poly(ethylene glycol), as solubilizing and shielding moiety, and a scFv antibody conjugated through the SNAP-Tag technology. The study provides the proof of principle that SNAP-tag technology can be used to generate drug-carrying nanoparticles efficiently modified with single-chain antibodies to specifically target and destroy cancer cells.