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Magnetic porous sugar-functionalized PEG microgels for efficient isolation and removal of bacteria from solution

: Behra, M.; Azzouz, N.; Schmidt, S.; Volodkin, D.V.; Mosca, S.; Chanana, M.; Seeberger, P.H.; Hartmann, L.


Biomacromolecules 14 (2013), Nr.6, S.1927-1935
ISSN: 1525-7797
Fraunhofer IBMT ()

Here, we present a new microparticle system for the selective detection and magnetic removal of bacteria from contaminated solutions. The novelty of this system lies in the combination of a biocompatible scaffold reducing unspecific interactions with high capacity for bacteria binding. We apply highly porous poly(ethylene glycol) (PEG) microparticles and functionalize them, introducing both sugar ligands for specific bacteria targeting and cationic moieties for electrostatic loading of superparamagnetic iron oxide nanoparticles. The resulting magnetic, porous, sugar-functionalized (MaPoS) PEG microgels are able to selectively bind and discriminate between different strains of bacteria Escherichia coli. Furthermore, they allow for a highly efficient removal of bacteria from solution as their increased surface area can bind three times more bacteria than nonporous particles. All in all, MaPoS particles represent a novel generation of magnetic beads introducing for the fir st time a porous, biocompatible and easy to functionalize scaffold and show great potential for various biotechnological applications.