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A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins

: Puettmann, C.; Kolberg, K.; Hagen, S.; Schmies, S.; Fischer, R.; Naehring, J.; Barth, S.


Immunology Letters 150 (2013), Nr.1-2, S.69-74
ISSN: 0165-2478
Fraunhofer IME ()

SNAP/CLIP-tag technology is a novel approach that allows tagged proteins to be covalently coupled to diverse labels, such as fluorochromes and particles, using a convenient and specific enzymatic reaction. A monoclonal antibody (mAb) that binds to the SNAP/CLIP-tag would be useful to determine labeling efficiency, and to achieve reproducible detection in a variety of experimental formats. We therefore generated the murine mAb M2D11 by standard immunization and hybridoma technology. M2D11 binds to both the SNAP- and the CLIP-tag in either the coupled or uncoupled configurations and can be detected in the context of ELISA, flow cytometry, immunohistochemistry and western blot. The new antibody increases the versatility of the SNAP-tag technology by enabling the detection of tagged proteins using conventional immunological methods and widely available secondary antibodies.