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Double-stranded RNA induces S100 gene expression by a cycloheximide- sensitive factor

: Voss, Andreas; Gescher, Kirsten; Hensel, Andreas; Nacken, Wolfgang; Zänker, Kurt S.; Kerkhoff, Claus


FEBS Letters 586 (2012), Nr.2, S.196-203
ISSN: 0014-5793
ISSN: 1873-3468
Fraunhofer IZI ()
cytokine burst; non-viral dsRNA; organotypic skin model; secondary response gene; tissue repair

Viral double-stranded RNA (dsRNA) and its synthetic analog polyI:C are recognized via multiple pathways and induce the expression of genes related to inflammation. In the present study, we demonstrated the polyI:C-induced gene expression of the damage associated molecular pattern (DAMP) molecules S100A8 and S100A9, while other S100 genes were not affected. Cycloheximide and Brefeldin A treatment revealed both the expression of S100A8 and S100A9 as secondary response genes and the involvement of polyI:C-induced cytokines herein. Several type I and type III interferons such as IFN, IL-20, IL-24, and IFN/IL-29 were expressed in response to polyI:C, however, they failed to induce S100A8 and S100A9 gene expression. These data indicate the involvement of the danger molecule S100A8/A9 in the resistance against viruses.