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Lung tumour growth kinetics in SPC-c-Raf-1-BB transgenic mice assessed by longitudinal in-vivo micro-CT quantification

 
: Rodt, Thomas; Falck, Christian von; Dettmer, Sabine; Hueper, Katja; Halter, Roman; Hoy, Ludwig; Luepke, Matthias; Borlak, Jürgen; Wacker, Frank

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Volltext urn:nbn:de:0011-n-2068985 (1.1 MByte PDF)
MD5 Fingerprint: 3d2c581cbd047133e236a6cc1e6afa23
Erstellt am: 21.8.2012


Journal of Experimental and Clinical Cancer Research 31 (2012), Nr.1, Art. 15, 7 S.
http://www.ncbi.nlm.nih.gov/pmc/journals/618/
ISSN: 1756-9966
ISSN: 0392-9078
Englisch
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer ITEM ()
micro-CT; transgenic mouse model; growth kinetics; lung; tumor

Abstract
Background: SPC-c-Raf-1-BxB transgenic mice develop genetically induced disseminated lung adenocarcinoma allowing examination of carcinogenesis and evaluation of novel treatment strategies. We report on assessment of lung tumour growth kinetics using a semiautomated region growing segmentation algorithm. Methods. 156 non contrast-enhanced respiratory gated micro-CT of the lungs were obtained in 12 SPC-raf transgenic (n = 9) and normal (n = 3) mice at different time points. Region-growing segmentation of the aerated lung areas was obtained as an inverse surrogate for tumour burden. Time course of segmentation volumes was assessed to demonstrate the potential of the method for follow-up studies.
Results: Micro-CT allowed assessment of tumour growth kinetics and semiautomated region growing enabled quantitative analysis. Significant changes of the segmented lung volumes over time could be shown (p = 0.009). Significant group differences could be detected between transgenic and normal animals for time points 8 to 13 months (p = 0.043), when marked tumour progression occurred.
Conclusion: The presented region-growing segmentation algorithm allows in-vivo quantification of multifocal lung adenocarcinoma in SPC-raf transgenic mice. This enables the assessment of tumour load and progress for the study of carcinogenesis and the evaluation of novel treatment strategies.

: http://publica.fraunhofer.de/dokumente/N-206898.html