Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Genome-wide transcriptome analysis of the plant pathogen Xanthomonas identifies sRNAs with putative virulence functions

: Schmidtke, Cornelius; Findeiß, Sven; Sharma, Cynthia M.; Kuhfuß, Juliane; Hoffmann, Steve; Vogel, Jörg; Stadler, Peter F.; Bonas, Ulla

Postprint (PDF; )

Nucleic Acids Research 40 (2012), Nr.5, S.2020-2031
ISSN: 0305-1048
ISSN: 0301-5610
ISSN: 1362-4962
Zeitschriftenaufsatz, Elektronische Publikation
Fraunhofer IZI ()

The Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is an important model to elucidate the mechanisms involved in the interaction with the host. To gain insight into the transcriptome of the Xcv strain 85-10, we took a differential RNA sequencing (dRNA-seq) approach. Using a novel method to automatically generate comprehensive transcription start site (TSS) maps we report 1421 putative TSSs in the Xcv genome. Genes in Xcv exhibit a poorly conserved-10 promoter element and no consensus Shine-Dalgarno sequence. Moreover, 14 of all mRNAs are leaderless and 13 of them have unusually long 5-UTRs. Northern blot analyses confirmed 16 intergenic small RNAs and seven cis-encoded antisense RNAs in Xcv. Expression of eight intergenic transcripts was controlled by HrpG and HrpX, key regulators of the Xcv type III secretion system. More detailed characterization identified sX12 as a small RNA that controls virulence of Xcv by affecting the inter action of the pathogen and its host plants. The transcriptional landscape of Xcv is unexpectedly complex, featuring abundant antisense transcripts, alternative TSSs and clade-specific small RNAs.