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Oligonucleotide and parylene surface coating of polystyrene and ePTFE for improved endothelial cell attachment and hemocompatibility
: Schleicher, M.; Hansmann, J.; Elkin, B.; Kluger, P.J.; Liebscher, S.; Huber, A.J.T.; Fritze, O.; Schille, C.; Müller, M.; Schenke-Layland, K.; Seifert, M.; Walles, H.; Wendel, H.-P.; Stock, U.A.
In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5N/m 2) and human blood serum for up to 96h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (P<0.05). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood.These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.