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Human biomonitoring of pyrethrum and pyrethroid insecticides used indoors

Determination of the metabolites E-cis/trans-chrysanthemumdicarboxylic acid in human urine by gas chromatography-mass spectrometry with negative chemical ionization
 
: Elflein, L.; Berger-Preiss, E.; Preiss, A.; Elend, M.; Levsen, K.; Wünsch, G.

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Journal of chromatography. B 795 (2003), Nr.2, S.195-207
ISSN: 0378-4347
ISSN: 1570-0232
Englisch
Zeitschriftenaufsatz
Fraunhofer ITEM ()
derivatization; GC; Pyrethrum; pyrethroid insecticide; Chrysanthemumdicarboxylic acid

Abstract
This work describes a gas chromatographic-mass spectrometric method employing negative chemical ionization (NCI) for the determination of E-cis/trans-chrysanthemumdicarboxylic acid (CDCA) in human urine used as a biomarker for the exposure to pyrethrum and/or certain pyrethroids in insecticide formulations applied indoors. Mixed-mode solid phase extraction was utilized for sample cleanup. Extraction recoveries ranged from 92 to 104% (2–9% R.S.D.). The acids were esterified with 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) allowing both their gas chromatographic separation and their sensitive mass spectrometric detection under NCI conditions. Detection limits of ca. 0.05 small mu, Greekg/l urine were achieved.

: http://publica.fraunhofer.de/dokumente/N-19161.html