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The aromatic peroxygenase from Marasmius rutola - a new enzyme for biosensor applications

: Yarman, A.; Gröbe, G.; Neumann, B.; Kinne, M.; Gajovic-Eichelmann, N.; Wollenberger, U.; Hofrichter, M.; Ullrich, R.; Scheibner, K.; Scheller, F.W.


Analytical and bioanalytical chemistry 402 (2012), Nr.1, S.405-412
ISSN: 1618-2642 (Print)
ISSN: 1618-2650 (Online)
Fraunhofer IBMT ()

The aromatic peroxygenase (APO; EC from the agraric basidomycete Marasmius rotula (MroAPO) immobilized at the chitosan-capped gold-nanoparticle-modified glassy carbon electrode displayed a pair of redox peaks with a midpoint potential of -278.5 mV vs. AgCl/AgCl (1 M KCl) for the Fe2+/Fe3+ redox couple of the heme-thiolate-containing protein. MroAPO oxidizes aromatic substrates such as aniline, p-aminophenol, hydroquinone, resorcinol, catechol, and paracetamol by means of hydrogen peroxide. The substrate spectrum overlaps with those of cytochrome P450s and plant peroxidases which are relevant in environmental analysis and drug monitoring. In M. rotula peroxygenase-based enzyme electrodes, the signal is generated by the reduction of electrode-active reaction products (e.g., p-benzoquinone and p-quinoneimine) with electro-enzymatic recycling of the analyte. In these enzyme electrodes, the signal reflects the conversion of all substrates thus representing an overa ll parameter in complex media. The performance of these sensors and their further development are discussed.