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High-resolution 1H magic angle spinning NMR spectroscopy of intact arctic char (Salvelinus Alpinus) muscle. Quantitative analysis of n-3 fatty acids, EPA and DHA

 
: Nestor, G.; Bankefors, J.; Schlechtriem, C.; Brännäs, E.; Pickova, J.; Sandström, C.

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Journal of agricultural and food chemistry 58 (2010), 20, S.10799-70803
ISSN: 0021-8561
Englisch
Zeitschriftenaufsatz
Fraunhofer IME ()

Abstract
The lipid and small metabolite profiles from intact muscles of Arctic char were investigated using 1H high-resolution magic angle spinning (1H HR-MAS) NMR spectroscopy. Not only the total n-3 fatty acid content but also the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents of the muscle were obtained from the 1H HR-MAS NMR spectra without pretreatment of the tissue or lipophilic extraction. A number of small metabolites could also be observed, where creatine/phosphocreatine, anserine and taurine were the most abundant. Thus, the use of 1H HR-MAS NMR led to simplified analysis techniques that can give direct information on the nutritional value of the fish.

: http://publica.fraunhofer.de/dokumente/N-174936.html