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2009
Journal Article
Titel
Surface selection, adhesion, and retention behavior of marine bacteria on synthetic organic surfaces using self-assembled monolayers and atomic force microscopy
Abstract
Adhesion of two marine bacteria Shewanella sp. strain T1 and Pseudoalteromonas sp. strain T8, on differently terminated alkanethiolate self-assembled monolayers on gold was investigated. The selected model surfaces-terminated by CH3, OH, NH2, COOH, OH-terminated oligo (ethylene glycol), and methyl-terminated oligo(ethylene glycol)-are characterized by contact angle measurement using water, methylene iodide, 1-bromonaphthalene, and formamide. Surface free energies were calculated. Cell counting of the two bacterial strains on the model surfaces after different times revealed differences between the two strains by at least one order of magnitude. For the different surfaces, the bacteria showed comparably small selectivity. Atomic force microscopy images of adhered bacteria showed very different fingerprints on the different surfaces.