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Peroxygenase based sensor for aromatic compounds

: Peng, L.; Wollenberger, U.; Kinne, M.; Hofrichter, M.; Ullrich, R.; Scheibner, K.; Fischer, A.; Scheller, F.W.


Biosensors & bioelectronics 26 (2010), Nr.4, S.1432-1436
ISSN: 0956-5663
ISSN: 1873-4235
Fraunhofer IBMT ()

We report on the redox behaviour of the peroxygenase from Agrocybe aegerita (AaeAPO) which has been electrostatically immobilized in a matrix of chitosan-embedded gold nanoparticles on the surface of a glassy carbon electrode. AaeAPO contains a covalently bound heme-thiolate as the redox active group that exchanges directly electrons with the electrode via the gold nanoparticles. The formal potential E°' of AaeAPO in the gold nanoparticles-chitosan film was estimated to be -(286±9) mV at pH 7.0. The heterogeneous electron transfer rate constant (ks) increases from 3.7 in the scan rate range from 0.2 to 3.0Vs-1 and level off at 63.7s-1.Furthermore, the peroxide-dependent hydroxylation of aromatic compounds was applied to develop a sensor for naphthalene and nitrophenol. The amperometric measurements of naphthalene are based on the indication of H2O2 consumption. For the chitosan-embedded gold nanoparticle system, the linear range extends from 4 to 40M naphthalene with a detection limit of 4.0M (S/N=3) and repeatability of 5.7% for 40M naphthalene.