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Near-infrared dye-loaded PLGA nanoparticles prepared by spray drying for photoacoustic applications

 
: Kohl, Y.; Kaiser, C.; Bost, W.; Stracke, F.; Thielecke, H.; Wischke, C.; Lendlein, A.; Kratz, K.; Lemor, R.

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International journal of artificial organs 34 (2011), Nr.2, S.249-252
ISSN: 0391-3988
Englisch
Zeitschriftenaufsatz
Fraunhofer IBMT ()

Abstract
Introduction: Nanoparticulate contrast agents are of great interest for diagnostic applications with high resolution in medicine. Here we present polymer-based degradable nanoparticles exhibiting a near infrared (NIR) absorption suitable for photoacoustic imaging. Methods: The nanoparticles were prepared by incorporation of indocyanine green (ICG) as NIR dye in poly[(rac-lactide)-co-glycolide] (PLGA) via an optimized spray drying process. By application of a multi-step centrifugation protocol, two different size fractions were achieved. The biocompatibitilty of the nanoparticles was tested in 2D cell cultures (human hepatocarcinoma cells and monkey kidney cells) using WST-1, BrdU and LDH assay. Results: Spherical particles were obtained with a good yield (> 81%), showing a high NIR-dye encapsulation efficiency (> 98%). By multi-step centrifugation, two different size fractions with a mean diameter of 640 nm and 390 nm were obtained. Cytotoxicity studies of the synthesized ICG-loaded PLGA particles were performed. No cytotoxic effect on metabolic activity, proliferation, or membrane integrity was observed. Conclusion: The high optical absorption at the relevant NIR-wavelength around 800 nm in combination with absence of cytotoxicity qualifies the ICG-loaded PLGA particles as promising candidates for degradable photoacoustic contrast agents.

: http://publica.fraunhofer.de/dokumente/N-163307.html