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Raman spectroscopy: A powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts

: Pudlas, M.; Koch, S.; Bolwien, C.; Hirth, T.; Walles, H.; Schenke-Layland, K.


Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Multiphoton Microscopy in the Biomedical Sciences XI : 23-25 January 2011, San Francisco, CA, USA
Bellingham, WA: SPIE, 2011 (Proceedings of SPIE 7903)
ISBN: 978-0-8194-8440-6
Paper 79032I
Conference "Multiphoton Microscopy in the Biomedical Sciences" <11, 2011, San Francisco/Calif.>
Fraunhofer IPM ()
biomedical optical imaging; bone; cellular biophysics; Raman spectroscopy

Bone-marrow mesenchymal stem cells (BM-MSCs) are a promising cell source for regenerative medicine. However, it is challenging to determine whether isolated BM-MSC populations are free of fibroblasts. We employed traditional methods and Raman spectroscopy to distinguish BM-MSCs and human dermal fibroblasts (hDFs). Although in vitro differentiation assays revealed the multipotent character of BM-MSCs, long culture periods are a major disadvantage. Using Raman spectroscopy, we could quickly distinguish between BM-MSCs and hDFs. Therefore we conclude that this method is sufficient for the rapid detection of fibroblastic contaminations in BM-MSC cultures.