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Modulares Produktionssystem zur automatischen Herstellung von dreidimensionalen Gewebestrukturen

Automatic production of biological tissue involves isolates donor tissue at a module; transferring isolated cells to second module propagates the isolated cells and third module to constitutes biological tissue
 
: Mertsching, H.; Heymer, A.; Hansmann, J.; Kaufmann, M.; Reis, C.; Huchler, R.; Traube, A.; Stallkamp, J.; Pretzsch, F.; Derichs, C.; Koropp, U.; Marx, U.; Pilgermann, S.; Saxler, J.; Stolzing, A.

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Frontpage ()

DE 102009022351 A: 20090515
DE 102009022351 A: 20090515
C12M0003
Deutsch
Patent, Elektronische Publikation
Fraunhofer IGB ()
Fraunhofer IPA ()
Fraunhofer IPT ()
Fraunhofer IZI ()

Abstract
(A1) Die Erfindung liegt auf dem technischen Gebiet des Tissue Engineering. Die Erfindung betrifft die automatische Herstellung von dreidimensionalen neu konstituierten biologischen Gewebestrukturen aus Einzelzellen oder Gewebeverbuenden. Die Erfindung stellt dazu ein automatisiertes modulares Produktionssystem und die Steuerung dazu bereit.

 

WO 2010130302 A1 UPAB: 20101130 NOVELTY - Automatic production of biological tissue newly constituted from animal or human donor tissue, involves providing the donor tissue at an input interface of first module which is specifically embodied to isolate from the donor tissue donor tissue cells of at least one cell type and to single out these cells (cell extraction module), to obtain singled-out cells of cell type that are isolated from donor tissue; transferring the isolated cells at a first interface of transition to a second module which is specifically embodied to propagate the respectively isolated cells of the cell type. DETAILED DESCRIPTION - Automatic production of biological tissue newly constituted from animal or human donor tissue, involves providing the donor tissue at an input interface of a first module which is specifically embodied to isolate from the donor tissue donor tissue cells of at least one cell type and to single out these cells (cell extraction module), to obtain singled-out cells of the at least one cell type that are isolated from the donor tissue; transferring the isolated cells at a first interface of transition to a second module which is specifically embodied to propagate the respectively isolated cells of the cell type (cell expansion module), so that in each case at least one cell group made up of propagated cells of the cell type is obtained from the isolated cells; transferring the cell group at a second interface of transition to a third module which is specifically embodied to newly constitute a biological tissue with the cell groups (tissue construction module), so that a newly constituted biological tissue is obtained from cell groups of at least one respective cell type; and providing the newly constituted biological tissue at an output interface. The newly constituted biological tissue contains at least one cell layer of fibroblasts and at least one cell layer of keratinocytes. INDEPENDENT CLAIMS are included for the following: (1) device for the automatic production of biological tissue newly constituted from animal or human donor tissue comprising a first module (cell extraction module) which is specifically embodied to isolate from the donor tissue donor tissue cell of at least one cell type and to single out these cells, comprising an input interface and a first transfer interface for transferring the isolated cells, second module (cell expansion module), specifically embodied to propagate the isolated cells of the in each case at least one cell type, which is connected downstream of the first module and shares with the first transfer interface, comprising a second transfer interface for transferring a group of propagated cells; and a third module (tissue constitution module), specifically embodied to newly constitute a biological tissue with the cell group, which is connected downstream of the second module and shares with the second transfer interface, comprising an output interface for ejecting the newly constituted biological tissue; and (2) computer program product for controlling the material flow in a device, where the first module has between the input interface and first transition interface a first material flow rate, the second module has between the first transition interface and second transition interface a second material flow rate and the third module has between the second transition interface and output interface a third material flow rate, where the program steps involves detecting at least one of the first, second and third material flow rates and/or state variables of the first, second and third modules; controlling at least one first, second and third material flow rate different, as a function of the detected at least one material flow rate and/or state variable in order to adapt the material flow rates of the modules to one another. USE - For automatic production of biological tissue newly constituted e.g. multilayered tissue from animal or human donor tissue e.g. biopsate of the human or animal body, multilayered skin tissue, or artificial multilayered skin tissue. ADVANTAGE - The method provides a complete automation that increased product safety and reproducibility when compared to common manual production processes in GMP-compliant laboratories. The modular construction also allows individual modules, such as the cell extraction module, the cell expansion module, or elements of the modules, such as the multifunctional pipette, the tissue grinder, to be operated individually in a flexible and stand-alone manner. As result the complete automation, the cell extraction process proceeds more rapidly than the manual laboratory process. This minimizes the risk of the biopsate drying out during the processing process.

: http://publica.fraunhofer.de/dokumente/N-148694.html