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Assessment of pulmonary function and serum substance levels in newborn and juvenile rats

: Lewin, G.; Hoymann, H.-G.; Fuhst, R.; Berger-Preiß, E.; Pohlmann, G.; Buschmann, J.


Reproductive toxicology 30 (2010), Nr.3, S.422-428
ISSN: 0890-6238
ISSN: 1873-1708
Fraunhofer ITEM ()
juvenile animal study; pharmacokinetic study; lung; plethysmography; nose only exposure; Verapamil; pulmonary function test; respiration

In the context of pharmaceutical development today, studies for pediatric drug approval are requested more and more often by the regulatory authorities. The developing lung represents a potential target in juvenile toxicity studies. Due to physiological differences in prenatal and postnatal development between humans and standard animal models, experimental methods have to be modified to assess pulmonary function, and basic data on respiratory parameters need to be provided. Daily nose-only inhalation exposure from postnatal days 4 to 21 using a model substance (verapamil HCl) and plethysmographic measurements between postnatal days 2 and 50 were performed noninvasively in conscious juvenile Wistar (WU) rats. The methods proved to be feasible and did not interfere with normal growth and development of the animals. Both techniques therefore permit new insights to support human neonatal risk assessment and therefore these animal models are suitable for regulatory studies.