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The primary transcriptome of the major human pathogen Helicobacter pylori

 
: Sharma, C.M.; Hoffmann, S.; Darfeuille, F.; Reignier, J.; Findeiß, S.; Sittka, A.; Chabas, S.; Reiche, K.; Hackermüller, J.; Reinhardt, R.; Stadler, P.F.; Vogel, J.

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Nature 464 (2010), Nr.7286, S.250-255
ISSN: 0028-0836
ISSN: 1476-4687
Englisch
Zeitschriftenaufsatz
Fraunhofer IZI ()
H. pylori; primary transcriptome; sequencing; small RNA; transcriptional start sites

Abstract
Genome sequencing of Helicobacter pylori has revealed the potential proteins and genetic diversity of this prevalent human pathogen, yet little is known about its transcriptional organization and noncoding RNA output. Massively parallel cDNA sequencing (RNA-seq) has been revolutionizing global transcriptomic analysis. Here, using a novel differential approach (dRNA-seq) selective for the 5' end of primary transcripts, we present a genome-wide map of H. pylori transcriptional start sites and operons. We discovered hundreds of transcriptional start sites within operons, and opposite to annotated genes, indicating that complexity of gene expression from the small H. pylori genome is increased by uncoupling of polycistrons and by genome-wide antisense transcription. We also discovered an unexpected number of similar to 60 small RNAs including the e-subdivision counterpart of the regulatory 6S RNA and associated RNA products, and potential regulators of cis- and trans-encoded target messenger RNAs. Our approach establishes a paradigm for mapping and annotating the primary transcriptomes of many living species.

: http://publica.fraunhofer.de/dokumente/N-125065.html