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A novel platform technology for the detection of genetic variations by surface plasmon resonance

: Mertig, M.; Bonsch, M.; Katzschner, B.; Voigt, J.; Sonntag, F.; Schilling, N.; Klotzbach, U.; Danz, N.; Begemann, S.; Herr, A.; Jung, M.


Institute of Electrical and Electronics Engineers -IEEE-; Institute of Electrical and Electronics Engineers -IEEE-, Sensor Council:
IEEE sensors 2009. Proceedings. Vol.1 : The Eighth IEEE Sensors Conference 2009. Christchurch, New Zealand, 25 - 28 October 2009
Piscataway, NJ: IEEE, 2009
ISBN: 978-1-4244-4548-6
ISBN: 978-1-4244-5335-1
Sensors Conference <8, 2009, Christchurch>
Fraunhofer IWS ()
Fraunhofer IOF ()
BioMEMS; biosensor; DNA; genetics; gold; infrared spectrometer; integrated optics; Lab-on-a-Chip; metallic thin films; micro optomechanical devices; microsensor; molecular-biophysics; optical sensor; surface plasmon resonance

We report on a novel approach to identify genetic variations based on the detection of specific polymerase-chain-reaction products by surface plasmon resonance. We use a recently developed, home-made spectrometer which exploits chips with integrated optics and microfluidics. The gold film at the chip surface is locally functionalized with single-stranded, thiol-modified probe DNA by applying a nanoliter dispenser. We discuss the chemical conditions for the achievement of maximum SPR signal strength and spot array density, and evaluate the SPR detection of selective binding of a set of 17 PCR products.