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Amino- and carboxy-functionalized nano- and microstructured surfaces for evaluating the impact of non-biological stimuli on adhesion, proliferation and differentiation of primary skin-cells
Structure-property relationships in biomineralized and biomimetic composites : Symposium held April 14 - 17, 2009, San Francisco, California, U.S.A.; Symposium KK, "Structure-Property Relationships in Biomineralized and Biomimetic Composites", held ... at the 2009 MRS spring meeting
Warrendale, Pa.: MRS, 2009 (Materials Research Society Symposium Proceedings 1187)
|Symposium KK "Structure-Property Relationships in Biomineralized and Biomimetic Composites" <2009, San Francisco/Calif.>|
Materials Research Society (Spring Meeting) <2009, San Francisco/Calif.>
|Fraunhofer IGB ()|
To gain basic insight into the impact of non-biological features on cells' behaviour, primary skin-cells (keratinocytes and fibroblasts) were cultured on amine-functionalized or carboxy-functionalized planar, nano- or microstructured surfaces. Sintered layers of silica nano- or microparticles were used to fabricate structures in the range of naturally occurring structure-sizes. Organo-chemical functionalization was achieved using organo-functional silanes. Primary skin cells isolated from human foreskin were cultivated on these interfaces. Keratinocytes showed significantly better adhesion and proliferation on amino-functionalized surfaces than on carboxy-functionalized surfaces. On amino-functional surfaces decreasing proliferation was detected from planar to microstructured surfaces. Fibroblasts tended to proliferate stronger on carboxylated surfaces. Actin and vinculin, a protein involved in the formation of focal adhesions, were equally expressed on all surfaces, proofingintact cell-substrate contacts.