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Intracellular potential measurements of adherently growing cells using microneedle arrays

 
: Tautorat, C.; Koester, P.J.; Held, J.; Gaspar, J.; Ruther, P.; Paul, O.; Cismak, A.; Heilmann, A.; Gimsa, J.; Beikrich, H.; Jonas, L.

Locascio, L.E. ; Chemical and Biological Microsystems Society -CBMS-, San Diego/Calif.:
Micro total analysis systems 2008. Proceedings of MicroTAS 2008. Vol.2 : Twelfth International Conference on Miniaturized Systems for Chemistry and Life Sciences, San Diego, CA, USA, October 12 - 16, 2008
San Diego, Calif.: CBMS, 2008
ISBN: 978-0-9798064-1-4
S.1777-1780
International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS) <12, 2008, San Diego/Calif.>
Englisch
Konferenzbeitrag
Fraunhofer IWM ()
electroporation; patch-clamp; membrane potential; Lab-on-a-Chip

Abstract
We present a new sensor chip system for intracellular potential measurements of adherently growing cells using micro-structured needle electrode (MNE) arrays. Existing methods for intracellular investigations are time-consuming, tedious or limited to the analysis of suspended cells. However, most biological cells grow adherently. To overcome these methodological limitations a novel technique, local micro-invasive needle electroporation (LOMINE) in MNE arrays, has been developed. LOMINE opens the cell membrane for introducing a MNE into the cytoplasm. This paper describes the fabrication process of the MNE-arrey chips and first cell electroporation experiments.

: http://publica.fraunhofer.de/dokumente/N-112865.html