Publica
Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten. Intracellular potential measurements of adherently growing cells using microneedle arrays
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Tautorat, C.; Koester, P.J.; Held, J.; Gaspar, J.; Ruther, P.; Paul, O.; Cismak, A.; Heilmann, A.; Gimsa, J.; Beikrich, H.; Jonas, L. | Locascio, L.E. ; Chemical and Biological Microsystems Society -CBMS-, San Diego/Calif.:
Micro total analysis systems 2008. Proceedings of MicroTAS 2008. Vol.2 : Twelfth International Conference on Miniaturized Systems for Chemistry and Life Sciences, San Diego, CA, USA, October 12 - 16, 2008
San Diego, Calif.: CBMS, 2008
ISBN: 978-0-9798064-1-4
S.1777-1780 |
| International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS) <12, 2008, San Diego/Calif.> |
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| Englisch |
| Konferenzbeitrag |
| Fraunhofer IWM () |
| electroporation; patch-clamp; membrane potential; Lab-on-a-Chip |
Abstract
We present a new sensor chip system for intracellular potential measurements of adherently growing cells using micro-structured needle electrode (MNE) arrays. Existing methods for intracellular investigations are time-consuming, tedious or limited to the analysis of suspended cells. However, most biological cells grow adherently. To overcome these methodological limitations a novel technique, local micro-invasive needle electroporation (LOMINE) in MNE arrays, has been developed. LOMINE opens the cell membrane for introducing a MNE into the cytoplasm. This paper describes the fabrication process of the MNE-arrey chips and first cell electroporation experiments.