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Production of highly ordered nanoporous alumina and its application in cell cultivation

: Höß, A.; Stäudte, A.; Thormann, A.; Steinhart, M.; Heilmann, A.

Chaikof, E. ; Materials Research Society -MRS-:
Designer biointerfaces. Online proceedings : March 24 - 28, 2008, San Francisco, California, USA ; 2008 MRS spring meeting ; Symposium CC
Red Hook, NY: Curran, 2008 (Materials Research Society Symposium Proceedings 1093E)
ISBN: 978-1-605-60847-1
Symposium CC "Designer Biointerfaces" <2008, San Francisco/Calif.>
Materials Research Society (Spring Meeting) <2008, San Francisco/Calif.>
Fraunhofer IWM ( IMWS) ()
nanoporous aluminum oxide; cell culture; HepG2; scanning electron microscopy

Nanoporous alumina membranes were utilized as cell culture substrates. Two different anodization processes were applied in order to obtain highly ordered, self-supporting nanoporous alumina membranes. The resulting membranes exhibit uniform nanopores with constant diameters having aspect ratios (pore depth:pore diameter) larger than 1000. Tentative experiments evaluating the cellular interaction of a hepatoma cell line (HepG2) with the nanoporous alumina membranes will be described. Furthermore, cell proliferation as well as the adhesion behavior of the cells on the porous substrates was investigated. Scanning electron microscopy (SEM) and focused ion beam (FIB) technology were applied to examine the cell morphology. The experiments revealed that the nanoporous membranes can be used as cel l culture substrates and that they have no adverse effects on cell proliferation and cell viability. Besides, cells on membranes with pore diameters larger than 200 nm developed small cell extensions (filopodia) which penetrated into the nanopores.